polyclonal rabbit antibody against sec61β Search Results


polyclonal rabbit antibody against sec61β  (Lampire Biological)
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Lampire Biological polyclonal rabbit antibody against sec61β
A point mutation that converts GFP to mGFP inhibits formation of OSER structures. (a) Immunoblot analysis of cells expressing unmutated or mGFP-Sec61γ lysates of equal amounts of cells untransfected (no DNA) or expressing either construct were loaded on the same gel and probed with anti-GFP or an antibody against native <t>Sec61β</t> as a control for equal loading. (b–d) Representative confocal micrographs of transiently transfected COS-7 cells expressing (b) mYFP-Sec61β, (c) mGFP-Sec61γ, and (d) C1(1-29)P450-mGFP reveal the absence of whorls, short karmellae, and loop structures. Other amorphous, often perinuclear, structures that are generally of much lower fluorescence intensity than cells expressing the unmutated GFP counterparts are visible. Bar, 5 μm.
Polyclonal Rabbit Antibody Against Sec61β, supplied by Lampire Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A point mutation that converts GFP to mGFP inhibits formation of OSER structures. (a) Immunoblot analysis of cells expressing unmutated or mGFP-Sec61γ lysates of equal amounts of cells untransfected (no DNA) or expressing either construct were loaded on the same gel and probed with anti-GFP or an antibody against native Sec61β as a control for equal loading. (b–d) Representative confocal micrographs of transiently transfected COS-7 cells expressing (b) mYFP-Sec61β, (c) mGFP-Sec61γ, and (d) C1(1-29)P450-mGFP reveal the absence of whorls, short karmellae, and loop structures. Other amorphous, often perinuclear, structures that are generally of much lower fluorescence intensity than cells expressing the unmutated GFP counterparts are visible. Bar, 5 μm.

Journal: The Journal of Cell Biology

Article Title: Formation of stacked ER cisternae by low affinity protein interactions

doi: 10.1083/jcb.200306020

Figure Lengend Snippet: A point mutation that converts GFP to mGFP inhibits formation of OSER structures. (a) Immunoblot analysis of cells expressing unmutated or mGFP-Sec61γ lysates of equal amounts of cells untransfected (no DNA) or expressing either construct were loaded on the same gel and probed with anti-GFP or an antibody against native Sec61β as a control for equal loading. (b–d) Representative confocal micrographs of transiently transfected COS-7 cells expressing (b) mYFP-Sec61β, (c) mGFP-Sec61γ, and (d) C1(1-29)P450-mGFP reveal the absence of whorls, short karmellae, and loop structures. Other amorphous, often perinuclear, structures that are generally of much lower fluorescence intensity than cells expressing the unmutated GFP counterparts are visible. Bar, 5 μm.

Article Snippet: The polyclonal rabbit antibody against Sec61β was prepared (Lampire Biological Laboratories) against the synthetic peptide PGPTPSGTNC (residues 2–10 plus a cysteine) of canine Sec61β conjugated to keyhole limpet hemocyanin using standard protocols.

Techniques: Mutagenesis, Western Blot, Expressing, Construct, Control, Transfection, Fluorescence